Genetic Characterization of Salmonella Typhimurium Isolates from Faeces of Children with Gastroenteritis Hospitalized in Baqiatollah-Azam Hospital, Tehran, Iran
Authors
Abstract:
Introduction: In Iran, invasive nontyphoidal Salmonella (iNTS) disease causes severe bacteremic illness among children <5 years old. The global yearly incidence of iNTS disease in children was reported to be in the 3.4 (range 2.1-6.5) million cases, (overall incidence 49 cases (range 30-94) per 100,000 population), the iNTS case-fatality ratio (CFR) of 20% yielded 681,316 deaths annually. Methods: The microarray analysis enables identification of the strains that have the 90kb Salmonella typhimurium virulence plasmid, presence or absence of the Salmonella pathogenicity islands (SPIs), adherence factors and other virulence determinants. Twelve isolates of S. typhimurium obtained from faeces of children with gastroenteritis were analyzed by microarray technique. Results: The virulence plasmid was present in 83.33% of isolates and all the isolates contained the SPI-4 and SPI-5. None of the strains had the cytolethal distending toxin, cdtB. All strains were positive for rck and mig-14. The adherence genes were present in all the strains in the range of 51.55% to 73.20% of the adherence genes interrogated in the microarray. Two strains were the least pathogenic S. typhimurium. Conclusion: Microarray analysis proved to be a valuable tool in confirmation of serotyping results and genetic characterization of S. Typhimurium.
similar resources
genetic characterization of salmonella typhimurium isolates from faeces of children with gastroenteritis hospitalized in baqiatollah-azam hospital, tehran, iran
introduction: in iran, invasive nontyphoidal salmonella (ints) disease causes severe bacteremic illness among children <5 years old. the global yearly incidence of ints disease in children was reported to be in the 3.4 (range 2.1-6.5) million cases, (overall incidence 49 cases (range 30-94) per 100,000 population), the ints case-fatality ratio (cfr) of 20% yielded 681,316 deaths annually. me...
full textCharacterization of Salmonella isolates from poultry sources in Iran
BACKGROUND: Salmonellosis is one of the most importantzoonotic diseases throughout the world. OBJECTIVES: Thepurpose of this study was to characterize a large collection ofSalmonella isolates from different poultry sources in Iran.METHODS: A total of 123 Salmonella isolates from differentpoultry sources were subjected to drug susceptibility test,hemolysin production, motility test, and plasmid ...
full textMultilocus Sequence Typing of the Clinical Isolates of Salmonella Enterica Serovar Typhimurium in Tehran Hospitals
Background: Salmonella enterica serovar Typhimurium is one of the most important serovars of Salmonella enterica and is associated with human salmonellosis worldwide. Many epidemiological studies have focused on the characteristics of Salmonella Typhimurium in many countries as well as in Asia. This study was conducted to investigate the genetic characteristics of Salmonella Typhimurium using m...
full textprevalence of atopic dermatitis in children with type 1 diabetes mellitus in southeastern of iran (kerman province): a case-control study
چکیده ندارد.
15 صفحه اولMolecular Characterization of a Salmonella Typhimurium Isolate from Caspian Pony
Typhoid disease or salmonellosis is a common sickness in horses. In several epidemiological studies inhospitalized horses, several serotypes of Salmonella often are predominant in nosocomial infections.Transportation, overcrowding, dehydration, oral antimicrobial therapy and infections are the risk factorswhich may activate latent or subclinical salmonellosis. In this study, t...
full textIncreased Isolation and Characterization of Shigella sonnei Obtained from Hospitalized Children in Tehran, Iran
Shigella flexneri has been the most frequent cause of shigellosis in children in Iran. To evaluate the changes in frequency of serogroups, 302 Shigella species were isolated in 2003 from hospitalized children, aged less than 12 years, with acute diarrhoea in Tehran, Iran. The number of collected S. sonnei, S. flexneri, S. boydii, and S. dysenteriae isolates was 178 (58.9%), 110 (37.4%), 10 (3.3...
full textMy Resources
Journal title
volume 3 issue 1
pages 29- 34
publication date 2015-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023